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Most vitamin E clinical trials were halted in the 1990s, with several trials concluding that vitamin E had no evident clinical therapeutic effect on BPD

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Assay of superoxide dismutase enzyme activity To measure the activity of superoxide dismutase enzyme, 1500 l of 100 mM phosphate buffer, 200 l of 0.2 mM methionine, 100 l of EDTA (3 mM), 900 l of distilled water and 100 l of sodium carbonate (NaCO 3 ) 1.5 Molar, and 100 l of riboflavin were mixed, and at the end, 50 l of the enzyme sample were added to each test tube