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The protein concentration in the lysates was measured using a BCA protein assay kit

The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

The importance of Glutathione cant be understated

They are particularly effective for dullness, uneven skin tone, and mild pigmentation

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Argininosuccinate synthase as a novel biomarker for inflammatory conditions
