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10.1038/s41568-022-00459-0 81 LeiJ.LuoD.XiongJ.LiM

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Cells were seeded at a density of 2.7 10 4 cells/cm 2 in culture flasks and were cultured in Williams Medium E (GIBCO, Darmstadt, Germany) containing 10% (v/v) fetal calf serum (FCS) (Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM L-glutamine (Thermo Fisher Scientific, Darmstadt, Germany), 5 g/mL insulin (Sigma-Aldrich, Steinheim, Germany), 50 M hydrocortisone-hemisuccinate (Sigma-Aldrich) and 1X antibiotic antimycotic solution (AAS) (Sigma-Aldrich)

Due to the heterogeneity of tumor tissues, the challenges posed by radioresistance are increasingly significant

10.1111/j.1541-4337.2011.00156.x 37 BuffensteinR.EdreyY

Separating doses throughout the day sacrifices this synergistic effect