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Biological analyses were performed on stool samples (16S rRNA sequencing) and blood serum (ELISA for oxytocin, inflammatory and neuroinjury markers), while behavioral outcomes were assessed using SRS, ABC and CGI

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All procedures were approved by the Animal Ethics Committee (AEC) of The University of Western Australia (RA/3/100/1613), with reciprocal approval from the AEC of Curtin University (ARE2019-4), and were performed in accordance with the principles of the National Health and Medical Research Council of Australia

Immunofluorescence staining The mice were deeply anesthetized and then euthanized by cervical dislocation