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Similarly, for the immunostaining of the M2 (F4/80+ CD206+) ovarian macrophages, PFA fixed ovarian sections were incubated overnight in a humidified chamber at 4 C with fluorescent-conjugated primary FITC anti-mouse F4/80 (123107, BioLegend), phycoerythrin-conjugated anti-mouse CD206 antibody (SC-376108, Santa Cruz Biotechnology), or an iso-FITC Rat IgG2a (400506, BioLegend) or phycoerythrin-conjugated goat anti-mouse IgG (ab97024, Abcam Inc.)

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Selected Research References: Gwyer D et al., Cell and Tissue Research (2019) Examination of BPC-157 in musculoskeletal soft tissue research models

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