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Recently, the use of a single-enzyme solution (trypsin, collagenase, or dispase) has been replaced by an increased number of commercially available kits that offer more standardized reagents and protocols requiring less operative expertise [142]

Key Advantages of LC-MS Lipidomics Enhanced separation resolution and speed : Modern ultra-high-performance liquid chromatography (UHPLC) systems with sub-2m particle columns dramatically improve separation efficiency and speed compared to traditional HPLC systems