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For measuring G q signaling, a plasmid mixture consisting of 200 ng LgBiT-inserted G q subunit, 500 ng G1, 500 ng C68S-mutant SmBiT-fused G2 (C68S), 200 ng cholinesterase-8A (Ric-8A) and 400 ng test GPCR in 200 L of Opti-MEM (Gibco) was transfected into cells using polyethylenimine (PEI)

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For 16.7 units, drawing to 17 gives you slightly more than 5mg

New Phytol 209:733745 Stochmal A, Kowalska I, Janda B, Perrone A, Piacente S, Oleszek W (2009) Gentisic acid conjugates of Medicago truncatula roots

Ameliorative effects of salicylic acid on mineral concentrations in roots and leaves of two grapevine ( Vitis vinifera L.) cultivars under salt stress

The presence of supporting nutrients matters significantly