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This step is the rate-limiting step of GSH synthesis and is regulated by cysteine availability

Indeed, MDA-MB-231 breast cancer cells and non-malignant retinal pigment epithelium cells (hTERT RPE-1) as well as human foreskin fibroblasts (HFF) also showed a significant increase in cell area and FA number upon aldolase depletion but not upon PFK or GAPDH loss, demonstrating that the differential impact of glycolytic enzymes on adhesions is conserved across cell types (Extended Data Fig

Physiology (Bethesda)

Metabolic competition in the tumor microenvironment is a Driver of cancer progression