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Using Ribonuclease A as model protein we show that inclusion of diMESNA during intein-mediated cleavage results in simultaneous on column refolding and thioester formation, providing a novel method for the production of correctly folded, disulfide-bridge containing proteins with a C-terminal thioester suitable for native chemical ligation

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& Vagner, S

The crosstalk between autophagy and senescence remains poorly defined

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