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Mouse breast tumors were dissected into 9.5mL DMEM/F12 media (C11330500CP, Gibco), the tumor tissues were chopped with razor blade into 1mm 3 small pieces, followed by digestion with collagenase type IV (1mg/mL) (#17104-019, Gibco), DNaseI (0.1mg/mL) (#LS002139, Worthington Biochemical) and hyaluronidase (0.2mg/mL) (#LS002592, Worthington Biochemical) at 37C for 1 h

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