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Epigenetic remodelling, such as elevated SET domain-containing 7 expression and increased H3 lysine 4 monomethylation at proinflammatory gene promoters (e.g., NF-B p65), sustains monocyte proinflammatory programming [51]

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Excess media was removed and replaced with 70% L15 media containing 10 M DCFH-DA and incubated at 20C for 30 min (32), after which the solution was removed, replaced with 10 g/ml Calcofluor White (CFW) to stain Bd for 5 mins (28), rinsed and replaced with 70% L15 media

Deloch et al., 2016), potentially biassing the commonly-held hypothesis that such regimens are better inducers of ICD