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For ratiometric measurements of the HyPer reporter the animals were imaged at a Zeiss Meta 710 confocal microscope at an excitation of 405 and 488 nm, while emission was detected at 516 nm
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Specifically, omega-3 fatty acids and coenzyme Q10 significantly enhanced sperm count and motility, while carnitines were particularly effective in improving sperm progressive motility
doi: 10.1093/humupd/6.2.122
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