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The cyclization step, involving the -amino group of lysine and -carboxy group of aspartic acid, was performed using an 8-fold excess of DCC as the coupling agent and 1-hydroxybenzotriazole (HOBt) as a racemization suppressant

doi: 10.5125/jkaoms.2013.39.5.207

[DOI] [PubMed] [Google Scholar] 5.Thompson JF, Scolyer RA, Kefford RF

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Draw the calculated volume of chilled bacteriostatic water into a sterile syringe
