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Furthermore, a study evaluated the targets of curcumin in colon cancer and found that it serves a role in ROS generation and autophagy induction (51)

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Lithium dodecyl sulfate sample buffer (Life Technologies/Thermo Fisher, Waltham, MA, USA) with dithiothreitol reducing agent (final concentration of 25 mm) was added to cell lysates before heating to denature proteins

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