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doi: 10.1038/s41598-017-18639-6 49 XieJShultsKFlyeLJiangFHeadDRBriggsRC

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then NHS (0.48 g, 4.08mmol) was added in 3 mL DMSO

After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)

We already knew that it varies in brightness, and we were confident that there are patchy cloud layers that rotate in and out of view and evolve over time, Boston University researcher Allison McCarthy explained in a statement on March 3