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doi: 10.1152/ajpendo.1988.255.6.E801

Clinical Update 7/31/2019 Fresh vs

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Methylmalonic acid and homocysteine levels can be measured to identify vitamin B12 insufficiency at its early stage if blood vitamin B12 levels are borderline

For RNA extraction, tissues were homogenized using a bead homogenizer in TRIzol Reagent, catalog # 15596026 (ThermoFisher Scientific), and RNA was extracted according to manufacturers protocols and purified using Econospin RNA Mini Spin Columns for RNA (Ephoch Life Sciences, Missouri City, TX, catalog #1940-250) following manufacturers protocols

Any elevation should trigger dose adjustment or discontinuation