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10.1038/s42255-021-00493-6 74 GuoS.ZhangY.-Y.PengJ.-J.LiY.-Q.LiuW.-N.TangM.-X.et al (2019)

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The connective tissue and the gall bladder were removed, and the liver was minced into small pieces and forced gently through a 200-mm-gauge stainless steel mesh using a sterile syringe plunger and then suspended in 50 ml Roswell Park Memorial Institute (RPMI)-1640 medium containing GlutaMAX-1, 25 mM HEPES and 10% FCS (pH 7.4)

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As shown in Table 1, the LE % values of CUR and miR155 were determined to be 6.75% and 3.63%, respectively, indicative of the efficient loading of CUR and miR155