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Isolation of cytosolic and mitochondrial fractions A total of 2 10 7 cells were collected and then mitochondrial separation agent (Beyotime Biotechnology Co., Ltd

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Similarly, a study performed using cortical neurons from rats showed that VPA treatment led to alterations in antioxidant defenses like GSSG and GSH and increases in the production of reactive oxygen species (ROS), mitochondrial damage, and lipid peroxidation, with an overall decrease in cell viability 17

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Moreover, NAD+ is also a key player in redox reactions, and its depletion is thought to contribute to the development of oxidative stress