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Compound C, an AMPK antagonist, weakened the analgesic effect of MOTS-c (9, 35)

This is thought to be due to anaemia that can occur as a result of vitamin b12 deficiency

GH and testosterone have well-documented additive effects on body composition testosterone drives muscle protein synthesis through androgen receptor activation, while GH promotes lipolysis, collagen synthesis, and tissue repair through separate pathways

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DSIP studies have not demonstrated tolerance development with repeated use

After, neurons were incubated in blocking solution containing donkey anti-mouse Alexa Fluor 488 minimal cross-reactivity antibodies (AF488, 1:500 dilution, Jackson ImmunoResearch 715-545-151) and vGlut1-STAR580 nanobodies (1:500 dilution, FluoTag-X2 N1602-Ab580-L, NanoTag Biotechnologies, RRID: AB_3076007 (custom-labelling order)) for 1 hour