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[DOI] [PubMed] [Google Scholar] Suthanthiran M, Anderson ME, Sharma VK, Meister A

A thermal cycling program similar to that described earlier 61 was followed, which included a denaturation step at 95 C for 10 min, followed by 40 cycles at 95 C for 15 s, an annealing step at 60 C for 30 s, and an extension step at 72 C for 30 s

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