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The treated cells were then washed with 1 PBS and fixed with 4% paraformaldehyde (Sigma-Aldrich)

Phosphoglycerate dehydrogenase (PHGDH) is the first enzyme branching from glycolysis in the serine biosynthesis which involved in one-carbon metabolism to supply S-adenosyl methionine (SAM) (120)

Additionally, it aids in energy production, helping to improve exercise endurance and overall vitality

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Target sample size and L-carnitine dose The published studies [9-11] used a sample size of 5060 patients in each group, and the administration of 900 mg of L-carnitine for 3 months led to a statistically significant improvement in the NCT-B results compared to the conventional treatment group (36.4% vs

Individuals at risk of choline inadequacy Daily intake recommendations are higher during pregnancy and lactation (see The AI), placing pregnant and lactating individuals at increased risk of choline inadequacy (25, 26)