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Prior to all experiments, MBP-FUS and MBP-FUS-GFP were centrifuged for 10 minutes at 21,000 g and 4C, and the clear supernatant was used to obtain protein concentration using Bradford assay

doi: 10.1038/nri2849 228 GuntiSNotkinsAL

2x higher than Core, adjustable up to 1000mg with $50 per additional 100mg ) Ascorbic Acid 40005000mg (22.5x higher than Core) Alpha Lipoic Acid (ALA) 100150mg (23x higher than Core) Glutathione 10001500mg (up to 2x higher than Core) Methylcobalamin (B12) 5mg (post-infusion push) Mini: 6090 min Plus: 24 hrs Max: 36 hrs NAD tiers take longer because NAD is infused slowly for comfort/tolerance

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Additionally, limited information on glycosylation and/or phosphorylation regulatory sites of the Gln transporters has been described

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