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Intact brains were extracted and post-fixed in 4% PFA overnight at 4 C then cryoprotected in 25% sucrose solution (pH 7.4) for 48 h
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You dont always know what youre getting
What I Used to Recover Cryotherapy Daily cold-therapy sessions to control inflammation, improve circulation, and trigger faster cellular repair
Julian Z, Mengesha B , McLemore MR, Steinauer J
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