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While protein disulfide exchange with a thiol can be catalyzed by PDI, some proteins contain a microenvironment in which thiolate (S ), which is far more reactive than is a thiol in both reaction with H 2 O 2 or disulfide exchange, is formed

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Then, cellular pellets were resuspended in 2 mL of assay buffer containing 20 mM Tris-HCl, 0.5 mM EDTA, 2% of ethanol at pH 7

As Pasciucco notes, water-based lubes are also safe to use with latex condoms, as well as condoms made from other materials like polyurethane and polyisoprene

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