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[DOI] [PMC free article] [PubMed] [Google Scholar] 148.Qian J, Klomsiri C, Wright MW, King SB, Tsang AW, Poole LB, Furdui CM

For the DUB assay, 30 l extract was used for each reaction and DTT and ATP were added fresh to a final concentration of 5 mM, followed by 0.5 M HA-Ub-VME

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Zheng M., Li Y., Jiang B., et al., Clinical Utility of Cerebrospinal Fluid CellFree DNA as Liquid Biopsy for Leptomeningeal Metastases in ALKRearranged NSCLC, Journal of Thoracic Oncology 14 (2019): 924932

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Thus, desulfuration reactions dominate when cysteine is deficient, whereas oxidative catabolism dominates when cysteine is in excess