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Description
Most patients with pernicious anaemia require lifelong treatment, and maintaining consistent therapy prevents recurrence of potentially serious complications
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Provided Materials and Reagent Materials Required But Not Provided Devices - Clean bench - Microplate Reader (610-630nm or 530-540nm filter) - Water Bath: 95 - Incubator: 37 - Centrifuge (8000g) - Sterilized tips: 20-200L, 100-1000L - Sterilized centrifuge tubes: 15mL, 50mL - Sterilized reaction tubes: 1.5mL / 2mL - Vortex - Sterilized syringe and 0.22m sterilized filter - Sterilized tweezers Reagent for native VB12 detection - Sterile water - 1% NaCH or KCN Solution:1g NaCN or KCN dissolved in 100mL redistilled water - 1M NaOH: 4g NaOH dissolved in 100mL redistilled water - Taka-Diastase from Aspergillus oryzae - Acetate buffer (pH 4.0): weigh 20.5g C 2 H 3 NaO 2 (Sodium Acetate) in a 1000mL beaker, add 950mL redistilled water and dissolve on a magnetic stirrer
Methyl B12 administration then fully normalized some hematopoiesis and blood patterns, improved the ratio between the B12 forms, and completely regenerated total B12 content
[DOI] [PubMed] [Google Scholar] 60.Zhou Z, Wang L, Song Z, Saari JT, McClain CJ, Kang YJ
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