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Immunohistochemistry and immunofluorescence Parts of each snap-frozen tissue block (including constrained and unconstrained explants) were sectioned at 5 m and 30 m thickness in the same cryostat, and post-fixed in acetone for 10 min at 20 C

& Sistla, R

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Experimental evidence supports these findings, where PHGDH knockdown reduces cellular D -2HG levels by 50% in PHGDH-overexpressing breast cancers, while overexpression increases D -2HG levels by 2-fold compared to wild-type PHGDH ( de novo biosynthesis, whereas knockdown significantly impairs proliferation, even in the presence of extracellular serine

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