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In particular, the intraperitoneal administration of K1K1 resulted in a strong neuroprotective activity on motor neurons in ALS animal models (Vallarola et al., 2020)

In many patients, these cells remain "primed," releasing cytokines (IL-1, TNF-) indefinitely

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The treated fibroblasts were then trypsinized and seeded at 2 10 5 cells/500 l of 1% FBS DMEM, penicillin, streptavidin in duplicate on the collagen gels and cultured for an additional 24 h at 37C in 5% CO 2