homocysteine and glutathione peroxidase-1 for Cardiovascular Risk Prediction: Results from the AtheroGeneStudy Modeling the Catalytic Cycle of

Narayanan et al., 2009

Sezen H, Kandemir H, Savik E, et al

Cancer Cell 182 YaoM

doi: 10.3969/j.issn.1004-0412.2022.11.024 11

Under conditions of oxidative stress, the liver exports oxidized glutathione into bile in a concentrative fashion, whereas under basal conditions, mainly reduced glutathione is exported into bile and blood

The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS