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75 mM Tris pH 8, 75 mM NaCl, 1 mM EDTA, 1 complete miniprotease inhibitor cocktail tablet per 10 ml, 5 mM sodium fluoride, 1 mM sodium orthovanadate, 5 mM -glycerol phosphate) and dropped in droplets out of pipette into liquid nitrogen
Statistical analysis Student's t test was used to compare results of healthy controls with Lyme borreliosis patients
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