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After a dialysis step to remove the glutathione present from the initial isolation, the sample is reapplied to the column and the sample of interest and thrombin is located in the column flow through

Therefore, based on the ranking in the hierarchy can be inferred that the functions of GPX2 may be more important than those of other GPXs

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Glutathione is synthesized in the cytosol of each cell by the action of two enzymes in an elegantly regulated system that allows it to be maintained at different homeostatic levels in different tissue types, with the liver, given its detoxification role, having the highest amounts

doi: 10.1038/309163a0