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Interestingly, the supplementation with Panaceo Sport positively influenced the intestinal wall integrity, which was witnessed through decreased concentrations of the tight junction modulator zonulin, a marker of increased intestinal permeability (Lamprecht et al., 2015)

The tissue homogenates and cell lysates were mixed with protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA, A32953 and A32957) to clear the lysates

IPEC-J2 cells were cultured in DMEM/F12 supplemented with 5% FBS, 5% PS, 1% ITS, and 5 ng/mL EGF in a 5% CO 2 atmosphere at 37C

The stained cells were immediately visualized under a fluorescence microscope (excitation: ~ 480 nm, emission: ~ 530590 nm)

Alexandria J Med 54(4):287293