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However, for ARPE-19 cells, ATCC recommends a mixture of Dulbeccos Modified Eagle Medium and Hams F12 Medium with 17.5 mM glucose

Standard water-based solutions may struggle to penetrate the scalps lipid barrier effectively

Acknowledgements We thank Sabine Lang (MHH, Department for Neurology) for technical support

The human CTR was modified to contain an N-terminal Flag tag epitope and a C-terminal 8 histidine tag epitope, each flanked by a HRV 3 C protease cleavage sites
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