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Accuracy of anemia diagnosis by physical examination
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Lysed cells were vortexed three times for 20 s each during incubation for 10 min at 4 C
[45], the effective induction of ALOX15 in human monocytes requires IL-4 concentrations at least 100-fold higher than the IL-4 plasma concentrations observed in atopic patients
After blocking the membrane in 5% skim milk, primary antibody was incubated over night at 4 C
200 l on day 0 and 100 l on day 4), and tissues were harvested on day 5 for analysis
