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They were maintained in IMDM medium (Invitrogen), and DMEM medium (Invitrogen) correspondingly, supplemented with 10% fetal bovine serum (FBS) (S711-001S, Lonsera) and 1% Penicillin-Streptomycin (SV30010, HyClone)

My energy returned, my skin looked healthier, and even my digestion improved

These calli were subcultured at 14-day intervals

Among these factors, p300 was the first protein identified with a writer function for histone lactylation

Declarations Authors contributions Made substantial contributions to conception and design of the study and performed data analysis and interpretation: Ugliono E, Rebecchi F Performed data acquisition, as well as provided administrative, technical, and material support: Seno E, Morino M Availability of data and materials Not applicable