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After incubating for 24 h at 37 C, the cells were washed with PBS and stained with Hoechst 33342 (Thermo Scientific), following the standard staining protocol provided by BioLegend
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On the one hand, a certain physiological level of ROS plays an important regulatory role, participating as specific signaling molecules in the regulation of metabolic processes, gene expression, and the functioning of the immune, endocrine and other physiological systems [4]
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