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Some patients include them in a broader wellness routine, and correcting a deficiency that was causing fatigue may indirectly support more consistent activity

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After 4 h incubation at room temperature, the sample concentration in the donor and acceptor wells were measured using a UV-Vis spectrophotometer (Beckman, DU640) set at the wavelength corresponding to the maximum absorption of each compound

At 0.5 mg/mL, the pink color is unmistakable

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