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For experiments with B-017-BPA (photoreactive amino acid BPA added to C-term of peptide), BPA was added as a custom amino acid (Name: BPA

33 Magnesium plays a significant role in CFE productivity as it is involved in ternary complex formation, ribosome assembly, and tRNA aminoacylation, among others, so the magnesium concentration is typically optimized for each individual extract

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[DOI] [PMC free article] [PubMed] [Google Scholar] 382.Levy J.M., Thompson J.C., Griesinger A.M., Amani V., Donson A.M., Birks D.K., Morgan M.J., Mirsky D.M., Handler M.H., Foreman N.K., et al