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gNG exhibits good ionization efficiency in negative ion mode because of the -conjugated system used for laser energy absorption and the nitrogen species doped as deprotonation sites for negative ion mode ionization

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Using a dissecting microscope (Leica M60), tissue punches of retina-choroid were carefully separated from the underlying sclera using curved forceps and paintbrush, and the retina carefully separated from the adjacent RPE-choroid in PBS

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doi: 10.1038/nature09784