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These are then oxidized into CO 2 and H 2 O through mitochondrial -oxidation, TCA cycle, and electron transfer

Even mild dehydration can affect concentration, memory, mood and reaction time

Brains were homogenized using lysis buffer for whole protein and mitochondrial protein extraction as previously described (Nguyen et al., Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes

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Willems L, Jacque N, Jacquel A, Neveux N, Maciel TT, Lambert M, et al