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Contrast enhancement of carotid adventitial vasa vasorum as a biomarker of radiation-induced atherosclerosis

Subcutaneous Injection (Research & Off-Label Use) Typical range: 250 mcg 2 mg per dose, depending on individual sensitivity

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PCR was performed with a 2 l cDNA mixture in a total volume of 35 l using Taq polymerase and 0.2 nmol/l of each forward and reverse primer (intron spanning, 5CCCCCGATAGCTCCTACAAC3 and 3AAGGCCTTCCTCATGCACT5, TRPV1 mRNA accession number NM_018727) after initial 95 C for 3 min over 35 cycles of 95 C for 30 s, 64 C for 30 s and 72 C for 45 s, finalized by 72 C for 7 min and a temperature hold at 4 C in a Techne TC-512 gradient thermal cycler (Staffordshire, UK), yielding a 295 bp product

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Donor [4% PIP (PI(3)P, PI(4)P, PI(3,5)P 2 , PI(4,5)P 2 , or PI(3,4,5)P 3 ) , 2% Rhodamine-PE, 94% DOPC], and acceptor [100% DOPC] liposomes were added at a 1:1 ratio