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Hossain F, et al
Although several steps in this proposed general mechanism still need detailed confirmation in ex vivo in vitro and in in vivo models through combined metallomic-metabolomic approaches [211,212] and through a systematic physico-chemical study of the involved molecular systems, the collected information allows us to design experimental approaches aimed at verifying the missing steps of the comprehensive model

Preparation of Lysate Cells or tissues to be screened are collected, lysed to prepare the lysate, and centrifuged to remove insoluble particles
Bernasocchi, T

& Millman, J.) 1115 (SciPy, 2008)