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C32E8.9 is required for the immune response activated by mitochondrial translation inhibition To untangle how C32E8.9 plays a role in mitochondrial translation-mediated lifespan extension without significantly altering UPR mt , we performed RNA sequencing on total RNA isolated from day 5 adult worms fed with mrps-5 RNAi, C32E8.9 RNAi, or the combination (Fig

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Briefly, SCD Day 10 erythroblasts were cultured at indicated conditions, and Cystine-FITC was added to cultured SCD erythroblasts at 100 M for 45 min, allowing uptake

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