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Beads were washed with low salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 150 mM NaCl), high salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 500 mM NaCl), LiCl buffer (250 mM LiCl, 1% NP40, 1% NaDOC, 1 mM EDTA, and 10 mM TrisHCl, pH 8), twice with TE buffer(10 mM TrisHCl, pH 8, and 1 mM EDTA), and finally with elution buffer (1% SDS and 100 mM NaHCO 3 )

Suppression of neuronal apoptosis and glial activation with modulation of Nrf2/Ho-1 and Nf-kb Signaling by Curcumin in Streptozotocin-induced diabetic spinal cord central neuropathy

It works by increasing serotonin levels, a natural substance in your brain that helps regulate your mood

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