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Immunohistochemistry The fixed tissues were processed using routine histological procedures and mounted on positively charged slides

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They also found that piperaquine, an anti-malarial drug, has the potential as a drug for ASD due to its ability to interact with ferroptosis-related genes [241]

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Samples were washed with DPBS and fixed with 3.7% Formaldehyde (F8775-25ML, Sigma-Aldrich) in DPBS for 7-10 min

Whitehead J, Lake B.