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The sorted cells, including hepatocytes, macrophages, and HSCs, were immediately pelleted by centrifugation (300 g , 5 min) and lysed in TRIzol reagent for qPCR analysis

reported that necroptosis-related genes, including MLKL , are significantly upregulated in gliomas [51]

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1 Introduction Numerous scientific studies have confirmed the role of diet in maintaining the proper functioning of the human body and in the prevention and treatment of various diseases, including obesity, diabetes, and cardiovascular disease ( 2 History of the ketogenic diet The earliest reports on the use of a low-carbohydrate diet to reduce the severity of symptoms, especially epileptic seizures, date back to antiquity ( In 1911, French doctors Guelpe and Marie published the first scientific report on the benefits of intermittent fasting in epilepsy (31, 32)