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After vortexing and incubation at 4C for 10 minutes, lysates were then centrifuged at 17,000g at 4C for 15 minutes and the supernatant was collected

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doi: 10.1007/978-1-0716-1162-3_13 126 ChenKZhangJZhangWZhangJYangJLiKet al