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Furthermore, we coordinate special handling for sensitive pharmaceutical shipments when the manufacturer or destination authority requires it

The gel was rinsed three times with Milli-Q water, stained for 30 min with Bio-Safe Coomassie Brilliant Blue (Bio-Rad Laboratories), and destained overnight in Milli-Q water

Finally, cells were resuspended in 100 l of ice-cold digitonin wash buffer in a heating block precooled in an ice-water bath

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For peptides, this molecular bumping and shaking can cause the amino acid chain to snap
