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LO2 and HepG2 cells were cultured in RPMI 1640 with 10% FBS at 37 in 5% CO 2

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Lipid measurements using LCMS For lipid extraction, samples were mixed with 20 C isopropanol (150 l per 5 l serum and 40 l per mg tissue), vortexed and immediately centrifuged at 16,000 g for 10 min at 4 C 15

JV, DK, MS, and ABB did the literature search and wrote the initial version of the manuscript