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Note: Note: The sample needs to be homogenized completely

In this work, 25 L GSH (2.5 mM) and 45 L CDNB (3.0 mM) were used as the substrate of the enzyme reaction, and pH 6.5 TrisHCl (20 mM) buffer was used as the enzyme reaction media

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doi: 10.1016/s0196-9781(03)00040-8

These electrolytes are crucial for proper muscle function, and their depletion can trigger cramps